MeasurementofCellAdhesionUnderStaticConditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adhesion in the convenient 96-well microtiter plate format is difficult due to the shear forces generated by washing the wells. This protocol introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity thereby eliminating uncontrolled shear f......閱讀全文
Measurement of Cell Adhesion Under Static Conditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell ad
細胞黏附和遷移
Cell Adherence Assay?(LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after the
碳水化合物分析
Carbohydrate Assay?(Hancock Laboratory) (Accessible only by IE) This protocol is used to determine the relative amounts of LPS CHO present in a giv
L-selectin-PNAd Interactions under Flow Conditions.
Purpose The main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecu
Cell to Cell Adhesion Signaling
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often invol
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates
Abstract The kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of?He
Dynamic Flow Assay in a Parallel Plate Flow Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Measurement of primary endothelial cell permeability to fluxes of dextran..
Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell
Dynamic Monitoring ofCellular Remodeling Induced bythe Transforming Growth1
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens
INTRODUCTION Flow cytometry is frequently used to assess nucleic acid content?in individual cells. Based on DNA content alone, however, cells?in the