CellCultureMediaandSolutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100X) L-glutamine12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AGFilter sterilize through a 0.22 祄 cellulose acetate filter and store up to 2 weeks at 4 degrees C.1X Cyclosporin media: (100 ......閱讀全文
Cell－Culture－Media－and－Solutions
Cell Culture Media and Solutions Dec. 18, 1990 R. Veile Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-gl
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 m
MEDIA－AND－SOLUTIONS－REQUIRED－FOR－ROUTINE－ES－CELL－CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
TISSUE－CULTURE－STOCK－SOLUTIONS－AND－MEDIA
MS MEDIUM FOR ARABIDOPSIS To?990?ml?H2O?add: Sucrose?...........?10.0??g MOPS?..............??0.5??g Agar?..............??8.0??g Adjust?pH?to?
Yeast－Media,－Solutions－and－Stocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
Bacterial－Media－Solutions－and－Stocks
3 agar?(200 ml)Add 6 grams agar to 200 ml deionized water. Autoclave to sterilize.1.6 agar?(200 ml)Add 3.2 grams agar to 200 ml deionized water.Autocl
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in
Raft－culture－media－(aka－Green’s－media)
Raft culture media (aka Green’s media) 3 parts DMEM (including glutamine or glutamax) 1 part Ham’s F12 5% FCS various supplements (not everybody
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department) For oocyte maturation and routine culture of mouse
Stock－solutions－for－tissue－culture
The kitchen makes Tris, TD, Tryp/TD, PBS, and VE.Tris?is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and i
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
細胞培養——培養基和試劑
·?????????Cell Culture Media and Solutions?(Donis-Keller lab)·?????????Cell Culture Media Formulations?(LTI)Comprehensive list of cell culture media,
Maintenance－of－Cell－Culture
Maintenance of Cell Culture Author:?Nanci Donacki Source:?Contributed by Nanci Donacki Date Added:?Tue May 14 2002
Bacterial－cell－culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
胚胎干細胞培養技術大全
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE Routine Culturing of ES Cells ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS MITOM
Virus－Infection－of－Fibroblasts
Procedure:? Day One? 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 c
Virus－Infection－of－Fibroblasts
Procedure:? Day One? 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 c
Cell－Suspension－Culture－of－Arabidopsis
實驗概要 Cell Suspension Culture of Arabidopsis? 主要試劑 10% (v/v) Household Bleach Callus Induction Medium? ? ? ?Gamborg's B5 Basal Medium? ? ? ?0.5
ES－Cell－Culture－and－Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood May 31, 1990 Rosalie Veile Purpose: Blood Samples can be stored frozen as a
InVitro－Adipocytes－Differentiation
Introduction Obesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an i
Method:－Reactivating－Cell－Lines－and－Cell－Growth－for－DNA－Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Working－Cell－Bank
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage . The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals pr
Fluorescence－Procedures－forthe－ActinandTubulin－Cytoskeleton－inFixed－Cells1
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
Method:－Preparation－of－Lymphoblastoid－Cell－Lines－for－Long－Term－Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage May 30, 1990 Rosalie Veile Purpose: To store cell lines in a form