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MaterialsGlass culture tubes with metal caps and labelsGrowth medium, from media room or customizedGlass pipette tubesParafilmEquipmentVortexerFireboy or Bunsen burnerMotorized pipetteMicropipettes and sterile tipsProcedureFor a typical liquid culture, use 5 ml of appropriate medium. The amount in each tube does not have to be exact if you are just trying to culture cells for their precious DNA.Streak an agar plate f......閱讀全文
實驗概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
實驗概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective eq
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a
實驗概要Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits.實驗原理The PureLink? HiPure Plasmid Purific
Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem CellsThe dissociation of adherent mesenchymal
實驗概要Plasmid DNA is isolated from large-scale (500 ml) bacterial cultures by treatment with alkali and SDS.主要試劑Buffers and SolutionsAlkaline lysis solu
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1: Tryp
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 μg/l tetracycline.Shake at 200 rpm and 37 °C untill
G. Bacterial cell maintenanceFour strains of E. coli are used in these studies: JM101 for M13 infection and isolation (4), XL1BMRF' (Str
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
I. TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often us
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make c
Plants offer a number of attractive benefits over conventional mammalian or bacterial cell culture systems for the production of valuable pharmace
INTRODUCTIONThis protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of im
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
Most plasmids can be adequately prepped by kits containing DNA binding columns. These columns do not do a great job of separating plasmid DNA from con
實驗概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of
實驗概要Sodium azide is a preservative used for inhibiting the growth of contaminants such as bacterial or fungus in antibody solutions. However, its
WESTERN BLOT PROTOCOLIn a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for dete
OverviewThis page details a electrotransformation protocol for Lactobacillus bacteria, specifically Lactobacillus delbruckii subsp
Purpose:Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet