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If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Media (Catalog # AR005, AR007). The protocol below has been used with the BG01V line of hES cells. Please note that other hES cell lines may require modifications of this protocol. Optimal culture conditions must be determined by the investigator for each hES lin......閱讀全文
IntroductionHuman embryonic stem (hES) cells are pluripotent stem cells derived from pre-implantation embryos that can be maintained and expanded in a
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
隨著人類基因組計劃（human genome project ）在2003年順利完成，基因組測序技術取得了長足的進步，這直接導致了每兆基因組成本的大幅下降以及檢測的基因組數量越來越多。人們對基因組的復雜性深感震驚，這也引導著測序技術的進一步發展。最近的一些突破性技術使得測序技術在更短的時間內可以
實驗概要Neural stem cells (NSCs) derived from human embryonic stem cells (hESCs) have the potential to help provide understanding for human ne
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用，研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說，2012年，Ellis等報道了基因與乳腺癌患者芳香酶抑制劑（aromatase inhibitor）治療法之間的關聯。他們指出突變，后果與診斷之間的關聯，同樣還有
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesa
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
實驗概要The ability to culture primary neurons under serum-free conditions facilitates tighter control of neuronal studies. Some serum-free me
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of a
實驗概要The protocols in this section describe the steps involved in differentiating neural stem cells (NSC) to neurons, astrocytes, and oligo
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem CellsIsolation and Transcription Profiling of Purified Uncultured Huma
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Materials and MethodsShort-term cell growth evaluationLonza? Poietics? human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad