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Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pellet from a liquid culture is touched with asterile pipette tip.Intact cells, a colony on a plate or liquid cultures which are stored at 4C for upto 3 months could still be used for this method.Wooden toothpicks should be avoided because they may interfere either release......閱讀全文
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· &nb
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
DNA克隆（主要內容如下）· General Procedure· PCR Clonin
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
芯片毛細管電泳具有進樣量少，靈敏度高，分析速度快等特點，非常適合法醫DNA-STR的快速檢驗。毛細管電泳芯片由于尺寸小，可施加較大場強，所以在幾秒鐘內就可完成對樣品的分離，微陣列毛細管電泳芯片可實現高通量檢測則成為目前學者研究的熱點。2002年Emrich CA等[31]報道的將高通量384孔毛
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
DNA轉化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab
6. Simultaneous digestion of the pUC vector with both enzymes in the presence of 3 units of Shrimp Alkaline Phosphatase (
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
ABSTRACTThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of t
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
實驗概要The E.Z.N.A.? SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole