Description RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure It is 3 days procedure.Day 1:1. Harvest the cells and centrifuge at 2000 rpm for 5-10 minutes and pour off the media.2. Wash the cell pellet with PBS and add 10 ml TriReagent solution.3. Incubate at Room Temperature for 5 min and during incubation pass the cell suspension through Pipette.4. Collect the solutions in ......閱讀全文
Protocol for Bioanalyzer RNA nano chippreparation of material12 samples per chipquantitative range 25–500 ng/μlquantitation accuracy 20%CV (for ladder
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
截至2019年12月13日,中國學者在Cell,Nature及Science在線發表了105篇文章(2019年的Cell已經全部更新完畢,而對于Nature及Science只剩下了一期,將分別會12月19日及20日進行更新),小編對于這些文章做了系統的總結: 按雜志來劃分:Cell 發表了30
截至2019年12月31日,中國學者在Cell,Nature及Science在線發表了186篇文章,其中生命科學領域有109篇,材料學有30篇,物理學有20篇,化學有12篇,地球科學有15篇。iNature團隊對于這些文章做了系統的總結: 按雜志來劃分:Cell 發表了31篇,Nature 發
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
人類免疫缺陷病毒(human immunodeficiency virus, HIV),即艾滋病(AIDS,獲得性免疫缺陷綜合征)病毒,是造成人類免疫系統缺陷的一種病毒。1983年,美國馬里蘭大學醫學院的Robert Gallo團隊[1][2]和法國巴斯德研究所的Luc Montagnier團隊
edited by Bruce A. Roe &n
實驗概要Dynabeads? Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purification, protein i
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl
實驗概要The method is a native chromatin immunoprecipitation protocol.主要試劑1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
RT-PCR AnalysisSolutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-10025 mM MgCl2use at a concentration of 1.5
腎臟疾病與糖尿病 糖尿病經常伴隨著腎病的發生[5]. 比如基因間lncRNA PVT1與二類疾病都緊密關聯。研究人員在II型糖尿病人的晚期腎病進程中發現了PVT1的基因變異。高糖處理可以誘導人的腎間質細胞中PVT1和纖連蛋白1、IV型膠原蛋白、TGFβ1、PAI1等因子的
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
人類免疫缺陷病毒(human immunodeficiency virus, HIV),即艾滋病(AIDS,獲得性免疫缺陷綜合征)病毒,是造成人類免疫系統缺陷的一種病毒。1983年,HIV在美國首次發現。它是一種感染人類免疫系統細胞的慢病毒(lentivirus),屬逆轉錄病毒的一種。HIV通過
3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannito
Nature Biotechnology 2009年2月封面文章:基于安捷倫寡核苷酸序列合成技術的液相序列捕獲系統 – 高通量平行靶向測序的最佳解決方案來自美國麻省理工學院和哈佛大學Broad研究院的研究人員,利用安捷倫(Agilent Technologies)卓越的寡核苷酸序列合成技術,合成
多功能單細胞顯微操作系統FluidFM BOT在活細胞提取中的應用由于細胞異質性的存在,單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后,提取內容物進行分析,然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點,但是操作苦難。如今一種全新使
由于細胞異質性的存在,單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后,提取內容物進行分析,然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點,但是操作苦難。如今一種全新使用FluidFM科技的技術新報道有望提供一種活細胞提取新型的簡易方
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
香港大學的 副教授一直從事著流感病毒的分子進化及流行病規律,SARS病毒的起源及進化等方面的研究,2005年曾由于一篇發表于《Nature》上的文章引起注目,去年刊登在《PNAS》上有關中國福建已經出現了一種新的H5N1禽流感病毒株——“類福建病毒”(Fujian-like,FJ-like)也引起了