RNA酶保護試驗((RNaseProtectionAssay,RPA)簡介
RNA酶保護試驗((RNase Protection Assay,RPA)是通過液相雜交的方式,用反義RNA探針與樣品雜交，以檢測RNA表達的技術。1。原理：雙鏈RNA（雜交的）能夠抵抗RNA酶的降解。2。應用：檢測RNA表達3。與Northern雜交和RT-PCR比較，RPA有以下幾個優點：1． 檢測靈敏度比Northern雜交高。由于Northern雜交步驟中轉膜和洗膜都將造成樣品和探針的損失，使靈敏度下降，而RPA將所有雜交體系進行電泳，故損失小，提高了靈敏度。2． 由于PCR擴增過程中效率不均一和反應“平臺”問題，基于PCR產物量進行分析所得數據的可靠性將下降，而RPA沒有擴增過程，因此，分析的數據真實性較高。3． 由于與反義RNA探針雜交的樣品RNA僅為該RNA分子的部分片段，因此，部分降解的RNA樣品仍可進行分析。4． 步驟較少，耗時短。與Northern雜交相比，省去了轉膜和洗膜的過程。5． RNA-RNA雜交體穩......閱讀全文
The－ribonuclease－protection－assay－(RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by th
Roche公司的RNase－Protection－Assay－(RPA)－protocol
Roche公司的RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes下載網址：http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no1_03/PDF/p22_23.pdf還有一份
RNA酶保護試驗((RNase－Protection－Assay,RPA)方法
一、試劑準備1． GACU POOL：取100mM ATP、CTP、GTP各2.78μl、100mM UTP 0.06μl，加DEPC H2O至100μl。2. 雜交緩沖液IPES 0.134g、0.5M EDTA（pH8.0）20μl、5M NaCl 0.8ml、甲酰胺8ml，加DEPC H2O至
RNA酶保護試驗((RNase－Protection－Assay,RPA)簡介
RNA酶保護試驗((RNase Protection Assay,RPA)是通過液相雜交的方式,用反義RNA探針與樣品雜交，以檢測RNA表達的技術。1。原理：雙鏈RNA（雜交的）能夠抵抗RNA酶的降解。2。應用：檢測RNA表達3。與Northern雜交和RT-PCR比較，RPA有以下幾個優點：1．
RNA酶保護實驗(RNase－Protection－Assay,RPA)簡介
簡介：RNA酶保護試驗(RNase Protection Assay，RPA)是通過液相雜交的方式,用反義RNA探針與樣品雜交，以檢測RNA表達的技術。1. 原理：雙鏈RNA（雜交的）能夠抵抗RNA酶的降解。2. 應用：檢測RNA表達3. 與Northern雜交和RT-PCR比較，RPA有以下幾個優
RNA酶保護實驗(RNase－Protection－Assay,RPA)簡介
簡介： RNA酶保護試驗(RNase Protection Assay，RPA)是通過液相雜交的方式,用反義RNA探針與樣品雜交，以檢測RNA表達的技術。 1. ?原理：雙鏈RNA（雜交的）能夠抵抗RNA酶的降解。 2. ?應用：檢測RNA表達 3. ?與Northern雜交和RT-PCR比較
RNA酶保護試驗((RNase－Protection－Assay,RPA)的優缺點
RNA酶保護試驗((RNase Protection Assay,RPA)是通過液相雜交的方式,用反義RNA探針與樣品雜交，以檢測RNA表達的技術。與Northern雜交和RT-PCR比較，RPA有以下幾個優點：1． 檢測靈敏度比Northern雜交高。由于Northern雜交步驟中轉膜和洗膜都將造
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
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Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
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In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
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The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al.,?J. Biol. Chem. 193: 265-
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This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dy