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CTCF is central to signaling pathways in immature B cells elicited by cross-linking the Ig BCR and stimulation with TGF?. Both stimuli result in induction of cell cycle arrest and apoptosis. BCR ligation stimulates a transient induction of MYC that leads to high level CTCF expression and feedback suppression of MYC transcription. BCR ligation also activates PTEN opposing PI3K activation of MYC. Pharmacologic inactiva......閱讀全文
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor o
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
Hypoxia (or low O2 levels) affects various pathologies. First, tissue ischemia, a variation in O2 tension caused by hypoxia/reoxygenation, can lead to
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
The G1/S cell cycle checkpoint controls the passage of eukaryotic cells from the first 'gap' phase (G1) into the DNA synthesis phase (S). Two
Presenilin-1 (PS1) is associated with gamma secretase activity that cleaves amyloid precursor protein (APP) and is implicated in Alzheimer'
Many transcription factors play essential roles in normal development by determining the proliferation and differentiation of cells. The coordinated t
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
NM_002128 Homo sapiens high-mobility group box 1 (HMGB1
以下是所有的看家基因的列表，大家在設計引物時，可以直接采用這些基因的序列，本表來自于Trends in Gentics上一篇文獻，內容全面，所有的看家基因均標注了基因銀行號（Genbank），可以直接到pubmed中查詢得出。引物設計可以推薦用primer 5，也可以直接與我們聯系設計，有關事宜，請
NM_033142 Homo sapiens chorionic gonadotropin, beta pol
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioall
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
對于所有多次分裂的細胞來說，維持染色體兩端端粒(telomere)的長度是至關重要的。一種稱作端粒酶(telomerase)的酶可使兩端得以延長，以抵消每次染色體拷貝所發生染色體縮短。端粒酶是細胞生存的必要條件，端粒酶功能喪失可導致干細胞自我更新障礙，從而引起諸如先天性角化不良、再生障礙性貧血和
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos r
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
The eukaryotic genome is packaged by histone and nonhistone proteins to form chromatin. The assembly of nucleosomesas well as compaction of nucleosoma
For ionisation to take place at all, chemical reaction between the sample and the&nbs
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix.
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
The PML nuclear bodies are ring-shaped nuclear substructures associated with the regulation of transcription, transformation, cell growth, and apoptos
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
來自北京大學生命科學學院、美國國立人類基因組研究所和加州大學生物系的研究人員，最近在利用逆轉錄病毒插入法引發斑馬魚全基因組范圍內基因突變的研究中取得重大進展。文章刊登于7月18日在線版《PNAS》。 研究人員采用其研制的一組技術，用假性逆轉錄病毒（pseudotyped retroviruses）
Many key signaling molecules are conserved from yeast to man. mTOR is a protein kinase involved in nutrient and growth factor signaling in humans that
雖然近年來肝癌診斷技術和治療水平不斷發展，但肝癌的早期診斷率依然較低。相當一部分患者在確診時已經是中晚期，失去了手術時機。常規放化療、肝動脈化療栓塞或分子靶向治療的效果均不理想。因此，肝癌的治療研究需要探索新的途徑。 2018年，12月18日，Cell Research在線發表了第二軍醫大學東
II. Labeling ProtocolThe procedure described below can be scaled down if desired. It is essential to perform all steps involving caged dyes under a sa