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1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagenase and the majority of the stromal cells, the tissue was rinsed and centrifuged. 4. The tissue was inoculated into a 60-mm tissue culture dish coated with collagen type I and grown in primary cell system supplemented with growth factors and ho......閱讀全文
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
表皮細胞廣泛遍布于身體，正常的表皮細胞較難轉染，尤其是使用基于脂質體技術的轉染試劑。我們使用電轉(Amaxa)方法轉染正常人的結腸表皮細胞并得到了65%的GFP標記細胞。非常感謝SignaGen,現在我們使用GenJet Ver II可以成功轉染正常人的結腸表皮細胞并且轉染效率顯著提高至75%。
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Digging through the literature, I've compiled a list of possible markers for various cell types, which I'll publish here for the benefit of an
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, rep
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
This diagram illustrates the opposing effects that Proepithelin (PEPI) and the elastase digest fragments, the epithelins (EPIa-g), have on epithelial
Over 1,000 papers and reviews have been written about the role of ceramide in the production of programmed cell death or apoptosis. Ceramide is a sphi
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
Maintaining the integrity of the gastrointestinal tract despite the continual presence of microbial flora and injurious agents is essential. Epithelia
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
2B. Transformation 1. Preparation of electrocompetent DH5a cells: autoclave 4 baffled 1 l
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
線粒體熒光探針信息大全 （Probes for Mitochondria）包括各種常用探針，如JC-1，JC-9，TMRM，TMRE等Mitochondria are found in eukaryotic cells, where they make up as much as 10% of th
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
CD1cPositive staining (normal): subset of normal peripheral B cellsPositive staining (disease): myeloid leukemias and some B cell malignanci