Isolationofpapillarycells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/streptomycin, and 0.35 g/l NaHCO3, pH 7.4, at 4°C. 2. After removal of the perinephric fat, the kidneys were sectioned along the anterior-posterior axis, ensuring that the papilla was left intact and attached to one of the two half-kidneys. 3. After......閱讀全文
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs) 1.?The human pulmonary arteries were opened to expose the endothelia
Isolation－of－T4－cells－from－xenograft－tumors
1 Put mouse down?2 Wash skin by dipping mice into ETOH?3 Remove tumor and put into 5ml PBS containing Fungizone and Penstrep?4 Swirl around to rinse t
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.? T
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
實驗概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104)?主要試劑SDS based extraction solution實驗步驟1. ?Harvest cells.1)?? Try
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells) Charles E. Krininger, III and Peter J. Hansen? Dept. of Animal Sciences, University of Florida Thi
Differentiate－ES－cells－into－glial－cells－and－neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells. ___________________ Day 1:?Tryp
Magnetic－Depletion－of－SSEA4+－Undifferentiated－Embryonic－Stem－Cells
實驗概要Human ?embryonic stem cells can be differentiated into neural-, mesenchymal ?and hematopoetic stem cells. This product is intended for the magneti
Differentiating－Neural－Stem－Cells－into－Neurons－and－Glial－Cells
實驗概要The protocols in ?this section describe the steps involved in differentiating neural stem ?cells (NSC) to neurons, astrocytes, and oligodendrocyte
Isolation－of－murine－splenocytes
Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the c
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
Isolation－of－colonic－epithelium
實驗概要 The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine. First
Chromosomal－DNA－Isolation
Chromosomal DNA Isolation METHOD: Grow cells in 2-5 ml broth to late log phase. Pellet 1-2 ml cells in microfuge. Resuspend cells in 400
Cosmid－DNA－Isolation
實驗概要 Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits. 實驗原理 The ?PureLink? HiPure Plasmid Purif
Isolation－Of－PCR－Products
實驗概要 Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. 實驗原理 The ChargeSwitch? Tech
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109?cells (OD600= 0.2 Dilute cells or scale up)
RNA－isolation－for－Microarray
Description?RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure?It is 3 days procedure.Day 1:1. Harvest the cells and cen
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation－of－bone－marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is:?1. Kill the rat and dissect out the fem
Fungal－DNA－Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
DNA－isolation－extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation