DetectionofMycoplasmabyCulture
AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). However there are some strains of mycoplasma that are non-cultivable (certain strains of Mycoplasma hyorhinis). The method is suitable for the detection of mycoplasma in both cell cultures and cell culture reagents and results are obtained within 4 weeks. Mycoplasma ......閱讀全文
Detection－of－Mycoplasma－by－Culture
AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho
Testing－for－Mycoplasma－by－Indirect－DNA－Stain－(Hoechst－33258－stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
Detection－and－Measurement－of－Radioactivity
Radioactive Decay Isotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are st
Detection－of－Glycoproteins－on－Blot
Detection of Glycoproteins on BlotSource:?Contributed by Sharad Purohit, Paller's LabReagentsSodium acetate Buffer (200mM, pH 5.5): Prepare? a 200
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
Detection－by－TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous?flagella - the bacterial cell has a single flagellaperitrichous?flagella - the bacterial cell has several flagella w
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Lactobacillus－culture
OverviewGeneral overview and guidelines on how to grow up a culture of LactobacillusMaterialsMRS broth (difco)MRS agar (difco)anaerobic conditionsProc
How－do－I－decontaminate－my－tissue－culture－(Invitrogen)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contami
Bespoke－Metal－Detection－Conveyor－Systems
Many metal detection applications do not fit into the scope of ? standard conveyor systems. For this reason, METTLER TOLEDO SAFELINE are able to
Protein－detection－onto－PVDF－membranes
2-D PAGE and electroblotting onto PVDF membranes have become widely used techniques for the characterization of proteins. Recent improvements have al
Detection－of－Intracellular－Antigens－by－Flow－Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
Culture－of－Endometrial－Explants
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen
Maintenance－of－Cell－Culture
Maintenance of Cell Culture Author:?Nanci Donacki Source:?Contributed by Nanci Donacki Date Added:?Tue May 14 2002
Bacterial－cell－culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in
TISSUE－CULTURE－ON－COVERSLIPS
I.?Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
Acetobacter－Xylinum－Culture
OverviewGeneral guidelines on how to grow up a culture of?Acetobacter xylinum, ATCC strain 53582 .Preparation of Acetobacter MediaTo prepare ～500 ml o
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐曉政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparation Wash embryos 1 x in 25% MMR Remove excess buffer Add 10 volumes "incubation buffer", i.e. 50 μl for 5 embryos Lyse the embryos
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION??Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Detection－of－BrdU－Incorporation－in－DNA－Synthesizing－Cells
Detection of BrdU Incorporation in DNA Synthesizing Cells?NOTE:?Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic an
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
牛支原體（mycoplasma）抗體酶聯免疫分析（ELISA）
?牛支原體（mycoplasma）抗體酶聯免疫分析（ELISA）試劑盒使用說明書本試劑僅供研究使用???????目的：本試劑盒用于測定牛血清，血漿及相關液體樣本中支原體抗體的含量。實驗原理：本試劑盒應用雙抗原夾心法測定標本中牛支原體抗體水平。用純化的牛支原體抗原包被微孔板，制成固相抗原，往包被單抗的
Immature－Embryo－Rescue－and－Culture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature
Cell－Culture－Media－and－Solutions
Cell Culture Media and Solutions Dec. 18, 1990 R. Veile Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-gl
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department) For oocyte maturation and routine culture of mouse