TestingforMycoplasmabyIndirectDNAStain(Hoechst33258stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks for detection by culture. However the staining of cultures directly with a DNA stain, results in a much-reduced sensitivity (~106cfu/ml). This may be improved by co-culturing the test cell line in the presence of an indicator cell line such as Vero (Prod.No. 84113001-1v1)......閱讀全文
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
用 Hoechst33258 測定DNA實驗
? ? ? ? ? ? 實驗方法原理 在緩沖液中勻漿細胞或組織，繼用超聲處理。以一定量的細胞懸液與Hoechst33258混勻，測定其熒光。 實驗步驟 材料非無菌：緩沖液：0.05
用 Hoechst33258 測定DNA實驗
實驗方法原理在緩沖液中勻漿細胞或組織，繼用超聲處理。以一定量的細胞懸液與Hoechst33258混勻，測定其熒光。實驗步驟材料非無菌：緩沖液：0.05mol/LNaPO4；2.0mol/L NaCl，pH7.4 , 含 2 X l0-3 mol/L EDTA 。Hoechst33258；緩沖液中，1
Indirect ELISA
實驗概要This protocol describes a method of indirect ELISA.主要試劑1.?Phosphate buffered saline (PBS) tablet: 10 mM phosphate buffer, pH 7.4, 150 mM NaCl
Indirect ELISA
實驗概要?In ?the indirect ELISA, the enzyme-antibody conjugate uses an antibody ?against the type of antibody that is used to detect the antigen, kind of
方案 21.3 用 Hoechst33258 測定DNA實驗
實驗方法原理在緩沖液中勻漿細胞或組織，繼用超聲處理。以一定量的細胞懸液與Hoechst33258混勻，測定其熒光。實驗步驟材料非無菌：緩沖液：0.05mol/LNaPO4；2.0mol/L NaCl，pH7.4 , 含 2 X l0-3?mol/L EDTA 。Hoechst33258；緩沖液中，1
Detection of Mycoplasma by Culture
AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho
Testing for Bacteria and Fungi
AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidium iodide)-red, dead
Gram Stain (+\-)
實驗概要細菌的革蘭氏染色技術實驗材料Colonies of bacteriaToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion實驗步驟1. Before staini