FlowCytometryofFibroblastNucleiforDNAcontent
MaterialsP.I. Solution: 4 mM Na3Citrate (0.118 g/100 mL)30 U/mL RNAseI (43 mg/100 mL)0.1% Triton-X100 (0.1mL/100 mL)50 μg/mL propidium iodide (5 mg/100 mL)ProcedureHarvest cells: Rinse with a subconfluent 10 mL dish with PBS (Ca++/Mg++ free). Cover with 1.5 mL 0.1% (2.5 mM) EDTA in PBS at 37oC x 10 min. Loosen cells by vigorous pipetting then transfer suspension to 1.5 mL Eppendorf tubes on ice.......閱讀全文
Flow Cytometry of Fibroblast Nuclei for DNA content
Materials P.I. Solution:? 4 mM Na3Citrate (0.118 g/100 mL) 30 U/mL RNAseI (43 mg/100 mL) 0.1% Triton-X100 (0.1mL/100 mL) 50 μg/mL propidium iodide
Flow Cytometry Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Yeast Cell Cycle by Flow Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
Intracellular Immunofluorescent Staining for Flow Cytometry
IntroductionA modification of the basic immunofluorescent staining and?flow cytometric analysis protocol?can be used for the simultaneous analysis of
Detection of Intracellular Antigens by Flow Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
Application Note: Qdot? Nanocrystal Conjugates in Flow Cytometry
實驗概要 Researchers today ?are trying to maximize the information that they get out of flow ?cytometry experiments by looking at more parameters in a s
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Intracellular Immunofluorescent Staining for Flow Cytometry-2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
流式細胞儀（Flow Cytometry）
1?流式細胞儀的概念及其發展歷史1．1 流式細胞儀的基本概念 流式細胞儀（flow cytonletry，FCM）是對高速直線流動的細胞或生物微粒進行快速定量測定和分析的儀器，主要包括樣品的液流技術、細胞的計數和分選技術，計算機對數據的采集和分析技術等。流式細胞儀以流式細胞術為理論基礎，是流體力學、
流式細胞術（Flow Cytometry, FCM）
流式細胞術（Flow Cytometry, FCM）是一種在功能水平上對單細胞或其他生物粒子進行定量分析和分選的檢測手段，它可以高速分析上萬個細胞，并能同時從一個細胞中測得多個參數，與傳統的熒光鏡檢查相比，具有速度快、精度高、準確性好等優點，成為當代最先進的細胞定量分析技術。流式細胞儀（Flow C