NoninvasiveHumanNuclearTransferwithEmbryonicStemCells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA1Corresponding author (sohyun@stanford.edu )INTRODUCTIONIn somatic cell nuclear transfer (SC......閱讀全文
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells Sohyun L. McElroy1 ?and? Renee A. Reijo Pera Center for Human Embryonic Stem Cell Re
Culture of Human Embryonic Stem Cells (hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Human Embryonic Stem (ES) Cell Protocols——Freezing Human ES Cells
? Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up). With a 5 ml pipet, gently pipet and scrape colon
Human Embryonic Stem (ES) Cell Protocols——Thawing Human ES cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)
實驗概要Directed ?differentiation of specific lineages has been a focal point in the ?field of human embryonic stem cell (hESC) research. Cell replacement
Human Embryonic Stem (ES) Cell Protocols—Splitting Human ES cells onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human Embryonic Stem (ES) Cell Protocols——Splitting Human ES cells on MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
Procedure for Culturing BG01V Human Embryonic Stem Cells
IntroductionHuman embryonic stem (hES) cells are pluripotent stem cells derived from pre-implantation embryos that can be maintained and expanded in a
Human Embryonic Stem (ES) Cell Protocols——Embryonic Bodies
Let human ES cells grow until the colonies are large and the cells are pretty piled up - about the time when you would normally split or even a day pa