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實驗概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treatment strategies for debilitating central nervous system (CNS) disorders. This protocol describes the in vitro expansion, passaging, and morphology of rat fetal NSCs in adherent or neurosphere suspension cultures.主要試劑Dulbecco’s Phosphate-Buffered Saline......閱讀全文
This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program, developed by Becton Dickinson. The
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot&n
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach s
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Care and Handling of Feeder Layer CellsSTO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing plasmid
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
實驗概要The LIVE/DEAD? Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
We use feeder-independent ES cell lines derived from the 129/Ola strain of mice (Nichols et al., Development 110, p.1341, 1990). These cells are easy
實驗概要The CellTrace? CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
實驗概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
實驗概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r