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Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live DIC analysis has largely superseded the use of visual fixed time point assays for analyzing microtubule dynamics, spindowns can still be very useful. Procedures for fixing and pelleting microtubules onto coverslips are described here.I. Solutions & SuppliesII. Spindo......閱讀全文
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin
分析測試百科網訊 SCIEX 是生命科學分析技術領域的全球領導者, 2018年1月30日在馬薩諸塞州弗雷明漢宣布推出新C100HT，用于生物制藥領域的定量糖基篩選。結合使用SCIEX的獲獎技術快速糖基化學（fast glycan chemistry，快速糖分析的樣品前處理技術）, C100HT
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
Contract Lab Using Flow Imaging Microscopy to Characterize Protein Aggregates 檢測機構使用流動成像顯微鏡來表征蛋白質聚集體Posted by Joyce BrownJoyce Brown發表Dan Berdovich kn
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
常用生物軟件（windows)全面介紹一、基因芯片1、基因芯片綜合分析軟件。 ArrayVision 7.0 一種功能強大的商業版基因芯片分析軟件，不僅可以進行圖像分析，還可以進行數據處理，方便protocol的管理功能強大，商業版正式版：6900美元。 Arraypro 4.0
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat
第一天(2013年6月6日)13:45-14:30 展商技術論壇 1： 會場：宴賓廳 II，2樓 議題：Cell-based assays and 3D cell culture：Your body is in 3D – Why is your cell cult
2013年5月16日-19日，由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是
For ionisation to take place at all, chemical reaction between the sample and the&nbs
Koshland Lab,Carnegie Institute http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.htmlDetermine the OD600 and correlate the cell
實驗概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
“香江學者計劃”學術委員會日前召開終評會議，2012年度”香江學者計劃”(以下簡稱”計劃”)共有64位內地優秀博士(名單見附件)獲得資助，將赴港與香港高等院校的導師開展為期兩年的合作研究。 ”計劃”在2011年首次資助44位內地優秀博士，已于今年3月
Determine the OD600 and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105, 1x105, and
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
Since most manufacturers aim primarily for compliance, there is little incentive to learn more about the formation or introduction of particles pr
October 22, 2015 - Giesen, GermanyWant to Prevent Costly Label-Mix-Up and Significantly Reduce Product Giveaway in Your Line?Easy task for the world&#
HYBRIDIZATION.Prehybridize blot at 65oC for ~3h in Church buffer containing 0.5mg/ml denature salmon sperm DNA (usually 14ml Church buffer plus 0.7ml
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
YWB per 10ml5mL 2M Sorbitol (if NZ arrested, add 40uL 1.5mg/mL0.336mL 1M K2HPO4 N2 to 4mL YWB)0.064mL 1M KH2PO44.6mL dH2OYWB, glycerol, PMSF5mL 2
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
MT Spindowns from ExtractsArshad DesaiNotes:The key variable in MT spindown experiments is ATP. Under high ATP conditions,conventional MAPs are select