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Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live DIC analysis has largely superseded the use of visual fixed time point assays for analyzing microtubule dynamics, spindowns can still be very useful. Procedures for fixing and pelleting microtubules onto coverslips are described here.I. Solutions & SuppliesII. Spindo......閱讀全文
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose g
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel elect
1. DNASIS 2.5 DNASIS for Windows 2.5版是日立軟件公司（Hitachi Sofeware Engineering Co.,Ltd.）97年推出的一個功能強大的序列分析軟件。包含有大部分分子生物學軟件的常用功能，可進行DNA，RNA，蛋白質序列的編輯和分析，
BioTechniques雜志日前評出了2015年最受歡迎的十篇技術文章，涵蓋了對PCR、DNA提取、無細胞蛋白表達等常規技術的改良，以及CRISPR/Cas9、Hi-Plex、微圖案化等新技術的應用。 1. Visual detection of isothermal nucleic aci
I. Useful Values1 mg/ml tubulin = 10 μM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t
I. Useful Values1 mg/ml tubulin = 10 μM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t
癌細胞的基因組中到處都是突變（單一核苷酸的突變），有些突變或會通過激活癌基因的表達或關閉抑癌基因的表達而誘發癌癥發生，然而可以說更重要的是在更大范圍內腫瘤細胞中發生著基因組的異常，比如，諸如這樣的細胞包含著異常數目的染色體（非整倍性），隨著腫瘤不斷進化，染色體的異常也會在毗鄰的癌細胞之間發生變化
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
尊敬的各位同行： 第2 屆大連國際色譜學術報告會及儀器展覽會包含第37屆國際高效液相色譜及相關技術會議(含儀器展覽會) (HPLC 2011 Dalian) 及第18屆全國色譜學術報告會及儀器展覽會(18th NSEC)，將于2011年10月8-11日在大連召開。 為使更多色譜工作者
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用，研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說，2012年，Ellis等報道了基因與乳腺癌患者芳香酶抑制劑（aromatase inhibitor）治療法之間的關聯。他們指出突變，后果與診斷之間的關聯，同樣還有
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It all
蛋白質組數據處理暨第三屆全國生物質譜學術交流會(第三輪通知) 為了積極促進我國蛋白質組學技術發展和應用、數據挖掘和生物質譜的經驗交流，由中國生物化學與分子生物學會蛋白質組學專業委員會、中國質譜學會生物質譜專業委員會和中國化學會分析化學委員會主辦，北京蛋白質組研究中心、復旦大學和蛋
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
芯片毛細管電泳具有進樣量少，靈敏度高，分析速度快等特點，非常適合法醫DNA-STR的快速檢驗。毛細管電泳芯片由于尺寸小，可施加較大場強，所以在幾秒鐘內就可完成對樣品的分離，微陣列毛細管電泳芯片可實現高通量檢測則成為目前學者研究的熱點。2002年Emrich CA等[31]報道的將高通量384孔毛
第五屆金屬組學國際研討會會議日程 Scientific Programme Wednesday, 9th Sept 2015 9:00 -21:00 Registration Wednesday, 9th Sept 2015, Ginkgo Hall
Target analysis by Parallel reaction monitoring (PRM)The targeted quantification and verification were carried out in 94 serum samples with 5~
Beginning 1. In this paper, we focus on the need for 2. This paper proceeds as follow. 3. The structure of the paper is as follows. 4. In this
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
CryoSAS 是半導體材料質量控制專用機型，主要應用在光伏和電子工業領域。CryoSAS: Cryogenic Silicon Analysis SystemThe Bruker Optics` Cryogenic Silicon Analysis System (CryoSAS) is a