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Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of ChromatinLEVEL IIMaterials Bovine or porcine brain0.25 M Sucrose containing 0.0033 M calcium acetate2.0 M Sucrose with 0.0033 M calcium acetate0.075 M NaCl with 0.024 M EDTA, pH 8.0Tris-HCl buffer, pH 8.0 with the following molarities0.05 M, 0.002 M, 0.0004 MTCA (Trichloroacetic acid)Tissue homogenizer......閱讀全文
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
近年來，深度學習等人工智能技術在圖像識別，自然語言處理等領域取得了令人矚目的成就。人工智能技術應用于生命科學，對生物信息多個領域產生了重大的影響。深度學習算法借助于生命科學大數據的飛速發展，從海量大數據中自動高效地提取特征進行學習，極大超越了依賴人工提取特征的傳統人工智能算法，在醫療圖像處理、結
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Chromosomal DNA Prep : cultured cells/tissue samples (Mike A Dyer)This protocol was developed for cultured cells but should be appropriate for di
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
翻譯后修飾蛋白質的定性和定量實驗 實驗步驟
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar