Cell adhesion is a fundamental feature of multicellular organisms including their defense mechanisms. In the later case in mammals, leukocytes play central role. They bind bacteria, parasites, viruses, tumor cells etc. Furthermore, their interactions with the endothelium are of special importance. Generally, lymphocyte adhesion and passage from the bloodstream to the lymphatic system occurs in the high endothelial ve......閱讀全文
B cell and T cell lymphocytes interact with a variety of cells as part of their immune function, circulating and homing in on specific stimuli in tiss
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
Cell adhesion is a fundamental feature of multicellular organisms including their defense mechanisms. In the later case in mammals, leukocytes play ce
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often involving interactions
Monocytes are a class of phagocytes involved in non-specific immune defense that develop into macrophages. In plasma, monocytes ingest and destroy bac
While T cells and B cells carry out the actions of antigen-specific immune responses, antigen-presenting cells called dendritic cells are required for
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
CXCR4 is a chemokine receptor in the GPCR gene family, and is expressed by cells in the immune system and the central nervous system. In response to b
Inflammation has several distinct components, including the localized response at the site of tissue injury or infection. Tissue injury stimulates the
FluidFM 測定細胞粘附力的應用隨著時間推移,越來越多的學者開始使用FluidFM 技術進行測定細胞粘附力。以下就近五年的具有代表性的應用進行總結。Cohen 等使用FluidFM 技術對MCF7-MCF10A、MCF7-HS5 的細胞粘附力進行了測定,并與以往的文獻進行對比,發現其數據與Hos
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC PlatesRonald L. Schnaar~Professor, Johns Hopkin
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
Cell Adherence Assay (LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
MN in Human Lymphocytes (method description)Lymphocyte isolation Lymphocytes were isolated using Ficoll-Paque density gradients
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adh
The strongest and unique “acute” environment induced by cryo-thermal therapy stimulated the most effective anti-tumor immune response As desc
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot&n
實驗概要試劑盒,操作簡單,稍有點貴,還可以接受。實驗材料blood實驗步驟Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
Method: Lymphocyte TransformationMay 30, 1990Rosalie VeilePrinciple:Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocyte