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Activation of the MAPK kinase pathway has been identified as a mechanism that integrins use to regulate gene expression leading to cell shape changes during cell spreading or migration Epithelial cells respond to extracellular matrix (ECM) cause integrin-mediated FAK phosphorylation that in turn phosphorylates the surrounding proteins (paxillin, Fyn/shc, and src) and leads to signal amplification. FAK also binds PI-3......閱讀全文
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
CXCR4 is a chemokine receptor in the GPCR gene family, and is expressed by cells in the immune system and the central nervous system. In response to b
Eukaryotic cells take up constituents of the extra-cellular environment and regulate the cell-surface level of membrane proteins via a recycling syste
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
Mitogen-activated protein (MAP) kinases are important players in signal transduction pathways activated by a range of stimuli and mediate a number of
The IL-2 receptor is a key component of immune signaling and is required for the activation, proliferation, and survival of T cells. This receptor is
GE HealthcareBenzamidine Sepharose™ 6B is p-aminobenzamidine covalentlyattached to Sepharose 6B by the epoxy coupling method.p-Aminobenzamid
SLRPs (Small leucine-rich proteoglycans) are extracellular molecules that bind to TGF-betas, collagens and other matrix molecules. In vitro, SLRPs wer
Ang II-stimulated Cardiac Dysfunction in ARKO Male Mice-We also evaluated the cardiac performance of these mice using echocardiography. Although l
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Four different members of the Sprouty protein family block the cellular proliferation and differentiation induced by several different growth factors,
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxy--ATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
Myocardial infarction damages heart tissue both during the initial ischemia and the subsequent reperfusion of tissues with oxygen. Corticosteroids can
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
While transcriptional regulation is often viewed as the most prevalent way extracellular signals to regulate gene expression, post-transcriptional reg
TNF acts on several different signaling pathways through two cell surface receptors, TNFR1 and TNFR2 (See TNFR1 and TNFR2 Signaling Pathways) to regul
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
4. 注釋( 1 ) 每個實驗均使用新鮮的 3 mol/L 甲醇- HCl 和硅烷化試劑。( 2 ) 要仔細識別蛋白質印跡，因為 WGA 既能識別 N-糖苷的 GlcNAc，也能識別 O-GlcNAc。( 3 ) 用于在硝酸纖維素印跡膜上封閉結合位點的溶液應避免糖蛋白污染。所以我們建議在這一步驟中使
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
The drug Gleevec (also known as imatinib mesylate or STI-571) was approved by the FDA in 2001 for the treatment of CML, chronic myeloid leukemia. Whil
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
實驗概要 Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most
實驗概要The E.Z.N.A.? Mollusc RNA Kit is designed for efficient recovery of total RNA greater than 200 nt from molluscs, arthropods, roundworms, flatw