ComprehensiveidentificationofnovelproteinsandNglycosylationsites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) software. The MS/MS spectra files were searched against the sequence database (72,672 entries) using in-house PEAKS software (version 6.0, Bioinformatics Solutions Inc.). The database was generated from protein sequences of Apis (downloaded April 2012),augmented with ......閱讀全文
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2?, Bin Han1?, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites二
ResultsIdentified novel royal jelly proteins ?To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digested wit
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites三
?Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites八
References1. Fujita T, Kozuka-Hata H, Ao-Kondo H, Kunieda T, Oyama M, Kubo T: Proteomic analysis of the royal jelly and characterization of the fu
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites六
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
a－pipeline－for－the－identification－of－intact－Nglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
Comparative－assessment－of－glycosylation－of－recombinant－human－...（七）
Figure 3 MS2 spectra of 2-AB-labeled N-glycan structures. Diagnostic ions are marked with corresponding fragment structures. (a) NeuGc1NeuAc1HexNA
a－pipeline－for－the－identification－of－intact－Nglycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
QTRAP代表文獻回顧
?生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 ????? QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合串聯液質聯用系統。QTRAP具有獨一無二的能力，可以運行蛋白
大規模蛋白質相互作用研究方法進展（四）
表1 蛋白質相互作用分析相關數據庫及網站? 網站 資源類型 網址? DIP? 蛋白質相互作用http: //dip.doe-mbi.uda.edu? INTERACT? 蛋白質相互作用http: //bioinf.man.ac.uk/interactpr.htm? ProNet? 蛋白質相互作用ht
幽門螺桿菌抗生素耐藥基因的分子檢測及分子對接分析
Abstract??摘要Aim:?To explore the mutation characteristics of H. pylori resistance-related genes to antibiotics of clarithromycin (CAM), levofloxaci
Comparative－assessment－of－glycosylation－of－recombinant－human－...（四）
Site-specific characterization of N-glycansFor intact N-glycopeptide analysis, chymotryptic digests of both hFSHs were subjected under UPLC equipped
Purification－of－MBP－(maLTosebinding－proteins)－Fused－Proteins
Express fusion proteins as per the?GST-fused protocol?up to Step 7 (Day 3). All steps in protein purification should be done at 4° C unless otherwise
蛋白質翻譯后修飾的驗證問題
Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（五）
??Figure 2. Proteomics analysis of serum glycoproteins from treated and untreated mice. 21 days after cell injection, mice underwent cryo-thermal or
A－novel－method－of－growing－fungi－for－DNA－extraction
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
Purification－of－GST－Fused－Proteins
Day 1Set up an overnight culture in 100 ml LMM broth or 100 ml terrific broth containing 100ul 100 mg/mlAmpDay 2Add 40-50 ml o/n culture to 1 lt terri
Synaptic－Proteins－at－the－Synaptic－Junction
The postsynaptic density (PSD) is a submembranous structure at the postsynaptic membrane mainly at the excitatory synapses. The neurotransmitter recep
A－novel－in－vitro－3dimensional－angiogenesis－model
1.?Human microvascular endothelial cells (HMVECs) with primary cell kits were cultured on collagen type I-coated dishes to 80% confluency, then ov
人工轉錄因子的部件——人類鋅指結構2
Table 2: Binding sites and identity of ZFPs used in?VEGF?activationWe then generated artificial transcription factors by fusing the three-finger domai
Crystallization－of－Kinesin－Family－Motor－Proteins
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human, rat andNeurospora?(Kull et a
Acetylation－(or－Succinylation)－of－Amino－Groups－on－Proteins
Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE:?Hanock and Benz. 1986. BBA. 860:699-707.PURPOSE:?Derivitization of amino groups t