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May 09, 2016 - Greifensee, SwitzerlandManual sample processing using flasks can introduce error into lab processes that lead to Out-of-Specification results. A new METTLER TOLEDO article explores 10 reasons to leave error-prone manual workflows behind and create greater accuracy and efficiency in routine sample preparation.METTLER TOLEDO’s new, free download “10 Reasons to Say Goodbye to Inaccurate Concen......閱讀全文
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
實驗概要This section provides a general protocol for automated isolation of genomic DNA from 10-20 μl blood samples in a 96-well format using
主要試劑1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remo
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxy--ATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
實驗概要This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions
EXERCISE G.2 LOWRY PROTEIN ASSAYMATERIALS0.15% (w/v) sodium deoxycholate72% (w/v) trichloroacetic acid (TCA)Copper tartrate/carbonate (CTC)20% (v/v) F
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblast
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl
實驗概要The protocol provides a method of hippocampal neuron cultures.主要試劑Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
實驗概要The method is a native chromatin immunoprecipitation protocol.主要試劑1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
實驗概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
實驗概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
一、實驗試劑 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g &n
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi