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Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay measures the secretion intensity of effector molecules released by immune cells inresponse to ex vivo antigenic stimulation, as well as the frequency of these responding cells. This assay is highly sensitive, quantitative, easy to use, and amenable to hig......閱讀全文
21. Add 100 μL of developing buffer to all wells using a multichannel pipette and incubate at room temperature for at least 2
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
實驗概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
IntroductionA modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous an
芬太尼是60 年代研制合成的阿片類藥物,主要用于麻醉,現已成為全球麻醉藥品增長最快的品種。在我國麻醉藥品中的市場份額亦逐步提高,市場銷售量在國內現有麻醉藥品中居第2位,已成為我國使用最多的麻醉藥品之一。近年來,國內外多應用靈敏、快速、高自動化的分析儀器實現對血漿中低濃度芬太尼的檢測,以滿足藥物動
隨著分析方法的飛速發展，無論是食品中有毒有害物質，還是環境中痕量元素的檢測，或者生物體內功能因子的分析，都迫切需要一種靈敏度高、快速準確、性能穩定的痕量分析方法。時間分辨熒光免疫分析技術（time-resolved fluoroimmunoassay，簡稱為TRFIA）是20世紀80 年代中期發展起
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
MitoTracker Green FM ProbeMitochondria in cells stained with nanomolar concentrations of our patented MitoTracker Green FM dye (M7514) exhibit bright
4 檢測p24抗原的方法臨床價值評判 4.1 p24抗原用于HIV1的早期診斷 成人HIV感染至抗體陽轉需要6個月時間［1］。p24抗原在感染早期，病毒大量復制而抗體產生很少時就能被檢測出來［4］。通過檢測血漿p24抗原，可以將“窗口期”減短至20 d，此時抗
編碼微球：分別用不同配比的兩種熒光染料將直徑5.6μm的聚苯乙烯微球（Beads）染成不同的熒光色，從而獲得多達100種經熒光編碼的微球。交聯探針、抗體或抗原：把針對不同檢測物的核酸探針、抗體或抗原以共價方式結合到特定熒光編碼的微球上。檢測反應：先把針對不同檢測物的、用不同熒光色編碼的微球混合，再加
液態芯片原理編碼微球：分別用不同配比的兩種熒光染料將直徑5.6μm的聚苯乙烯微球（Beads）染成不同的熒光色，從而獲得多達100種經熒光編碼的微球。 交聯探針、抗體或抗原：把針對不同檢測物的核酸探針、抗體或抗原以共價方式結合到特定熒光編碼的微球上。 檢測反應：先把針對不同檢測物
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
6. Simultaneous digestion of the pUC vector with both enzymes in the presence of 3 units of Shrimp Alkaline Phosphatase (
AbstractTumor genotyping can provide a useful guide to drive clinical trials, inform treatment options, and predict patient outcomes1. This is due, in
實驗概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
Note: Samples to be used within 5 days can be stored at 4°C, besides that, samples must be stored at -20°C (assay ≤1 month) or -80°C(assay≤2 month
五、使用儀器發表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu