WIKI資訊
Characteristics of the procedurePreparation of isolated nuclei - procedurePreparation of radioactive labeled nucleiMaterial Characteristics of the procedure:Pretreatment of epithelial cells with 20 礛 Cytochalasin B for 30 min in RPMI prior to harvesting results in the reduction of cytoskeletal microfilaments and the easier release of the nuclei from the surrounding cytoskeleton during cell lysis. For the prepara......閱讀全文
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
7 展望選擇性微電極技術能用于直接并靈敏地觀察植物體對礦質元素的需求，研究者可利用選擇性微電極技術進行對植物某種離子或高或低的吸收的品種的篩選，還可制定出與植物需求相適應的環境的營養水平；能及時準確地探測到的光、溫、水澇、鹽分引起的植物體離子或分子信息的微小變化，能成為預測植物受到逆境脅
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our unde
多功能單細胞顯微操作系統FluidFM BOT在活細胞提取中的應用由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使
由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使用FluidFM科技的技術新報道有望提供一種活細胞提取新型的簡易方
實驗概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
4. 注釋( 1 ) 每個實驗均使用新鮮的 3 mol/L 甲醇- HCl 和硅烷化試劑。( 2 ) 要仔細識別蛋白質印跡，因為 WGA 既能識別 N-糖苷的 GlcNAc，也能識別 O-GlcNAc。( 3 ) 用于在硝酸纖維素印跡膜上封閉結合位點的溶液應避免糖蛋白污染。所以我們建議在這一步驟中使
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl
實驗概要The method is a native chromatin immunoprecipitation protocol.主要試劑1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
This procedure describes a convenient method for the isolation of crude nuclear pellets from N. crassa. The method, an adaptation of the one deve
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vei
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscie