AnUltrafastmethodofDNAextractionfromNeurospora
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) while giving excellent yield and size of DNA, is somewhat cumbersome and also results in the occasional sample that proves to be uncuttable. We report here a simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to fini......閱讀全文
An－Ultrafast－method－of－DNA－extraction－from－Neurospora
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) w
DNA－Extraction－from－Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
DNA－Extraction－from－Tissue
實驗概要DNA extraction from tissue.主要試劑Extraction buffer100 mM Tris-HCl (pH 8.0)?????100 mM EDTA (pH 8.0)?100 mM Na-Phosphate (pH 8.0)???1.5 M NaCl1% CTAB
Fast－and－reliable－miniprep－RNA－extraction－from－Neurospora－crassa
We have developed a method for isolating high quality total RNA from?N. crassa?mycelia that reliably yields large quantities. It is possible to extrac
A－novel－method－of－growing－fungi－for－DNA－extraction
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
DNA－EXTRACTION－FROM－MICRODISSECTED－PARAFFIN－SECTIONS
This is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologi
DNA－Extraction－from－Frozen－Tissue－Sections
Tissue collection, storage, microdissection, sectioning: See separate protocol.Tissue handling: Note that all fresh tissue should be handled as BioSaf
A－safe－method－of－extracting－DNA－from－Coccidioides－immitis
Human-pathogenic fungi such as Coccidioides immitis and Histoplasma capsulatum must be handled in Biosafety level 3 containment facilities which make
Chromosomal－DNA－Extraction－from－Grampositive－Bacteria
Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed for?Listeria monocytogenes?but has worked well with othe
Extraction－of－DNA－From－Plants－Using－Plant－DNAzol?－Reagent
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
Extraction－of－RNA－from－Fibrous－tissues
實驗概要 E.Z.N.A.? ?MicroElute? Total RNA Kit provides a rapid and easy method for the ?isolation of up to 50 ug of total RNA from small amount of cultu
Extraction－of－RNA－from－Frozen－Sections
RNA Extraction from Frozen Tissue Sections?Tissue Handling:?Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear
Protein－extraction－from－whole－tissues－for－IEF
Modified from that of Jay Thelen - University of Missouri-Columbia Phenol extraction followed by methanolic ammonium acetate precipitation - an effe
Lipid－analysis：EXTRACTION－OF－LIPIDS－FROM－LIPOPHORIN
Lipid analysisEXTRACTION OF LIPIDS FROM LIPOPHORIN?Since lipids are hydrophobic, they are better soluble in organic solvents than in water. Because th
Method:－Removal－of－Yeast－Contamination－from－Lymphoblast－Cultures
Purpose:This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when s
DNA抽提
DNA抽提（主要內容如下）·???Working with DNA·???DNA Extraction from Bacteria and Other Organisms·???DNA Extraction from Cell and Tissue·???Mitochondria DNA Isola
細菌的核酸抽提
DNA Extraction·?????????DNA Extraction from Bacteria?(Julie B. Wolf,UMBC)Phenol/chloroform method·?????????DNA Extraction From Bacteria (Triton Method
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood May 31, 1990 Rosalie Veile Purpose: Blood Samples can be stored frozen as a
Multiplex－PCR－Method－to－Discriminate－Artemisia－iwayomogi－from－Other－...
Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi , commonly referred to as “Haninjin,” is o
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
DNA－isolation－extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wi
Fungal－Genomic－DNA－Extraction
Overview High throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liqu
DNA－EXTRACTION－PROCEDURE－－GENERAL
Grow cells overnight in 500 ml broth medium. Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA. Freeze ce
Fungal－Genomic－DNA－Extraction
實驗概要 This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction. Hi
Automated－Genomic－DNA－Extraction
實驗概要This section ?provides a general protocol for automated isolation of genomic DNA from ?10-20 μl blood samples in a 96-well format using the Charge
Genomic－DNA－Extraction－－PureLink?
實驗概要The ?PureLink? Genomic DNA Purification Kit allows rapid and efficient ?purification of genomic DNA. The kit is designed to efficiently isolate ?g
Fungal－Genomic－DNA－Extraction
OverviewHigh throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liquid
Small－scale－DNA－preps－for－Neurospora－crassa.
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an av