WIKI資訊
Protocol of Northern blot質粒的轉化和擴增質粒的鑒定目的基因片段的切割3.1樣品雙酶切(175μl水解體系)DW 115μlBuffer B(10×) 17.5μlBaM H 15μlPst I 17μlDNA(MMP-9) 16μlBSA 4.5μl37℃水浴，3h。3.2制膠(1.5%Agarose)0.6g Agarose+40ml TAE(1×)3.3電泳175μl樣品+35μl loading buffer(6×)Marker 5μl +DW 20μl + 5μl loading buffer(6×)3.4目的基因片段的回收（按照北京鼎國生物發展中心的DNA純化回收手冊進行）3.4.1 切取含DNA片段的瓊脂糖（100mg-300mg）搗碎按重量比1：3（DNA片段：溶液B）加入溶液B。3.4.2 50℃水溶10分鐘，直至膠完全溶化，其間旋渦振蕩三次，瓊脂糖必須完全溶化，如果體積大于500μl，......閱讀全文
DNA標記（主要內容如下） DNA Labeling by Nick Translation Random Primed Labeling End-Labeling Purification of Labeled
Serial Analysis of Gene Expression (SAGE) SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital
· What's Differential Display (GenHunter)Introduction to differential display technique·
實驗概要The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a
實驗概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible
Solublization of RNA in Formamidecontributed by James McCaughern-Carucci, Yale UniversityResuspending RNA in Formamide (as reported by Chomczynski et
Western免疫印跡（Western Blot）是將蛋白質轉移到膜上，然后利用抗體進行檢測。對已知表達蛋白，可用相應抗體作為一抗進行檢測，對新基因的表達產物，可通過融合部分的抗體檢測。一、原理與Southern或Northern雜交方法類似，但Western Blot采用的是聚丙烯酰胺凝膠電泳，被
Western免疫印跡（Western Blot）是將蛋白質轉移到膜上，然后利用抗體進行檢測。對已知表達蛋白，可用相應抗體作為一抗進行檢測，對新基因的表達產物，可通過融合部分的抗體檢測。 本文主要通過以下幾個方面來詳細地介紹一下Western Blot技術: 一、原理 二、分類