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Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next morning.Spin down cells in 50 ml sterile conical tubes for 10 min. Centrifugation steps are performed at 4 degrees; all other steps are carried out at rm. temp. unless otherwise specified.Resuspend cells in 10 ml water and spin down cells.Resuspend cells in 3 ml of 0.9 M......閱讀全文
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events i
Southern雜交One important thing for transfer:the weight of the object resting on top of the blotting apparatus should not exceed the weight equal to a 5
FastDNA 試劑盒可從廣闊的各種來源中快速有效地分離出 genomic DNA，配套 FastPrep 儀器使用。動植物組織、細菌、海藻、真菌和其他許多標本，在 40 秒內容易被裂解。這個 benchtop 設備是一種專利性的垂直角度運動，這種運動
Human-pathogenic fungi such as Coccidioides immitis and Histoplasma capsulatum must be handled in Biosafety level 3 containment facilities which make
不同 gDNA 起始量的電泳疊加圖顯示，使用 2100 生物分析儀 (A) 和 4200 TapeStation (B) 系統，文庫片段分子量分布（圖 5）呈現出不同的彌散條帶曲線。50 ng 的最佳 gDNA 起始量可得到平均分子量在 600–1000 bp 之間的文庫曲線。最小的 gDN
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel elect
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose g
CRISPR已經應用于包含人類，小鼠，酵母，水稻等各個物種中，取得了空前的成功。就在近期，Nature Biotechnology 雜志連續推出了4篇CRISPR技術的進一步升級應用，同時也進一步拓寬了CRISPR技術應用范圍，尤其是為臨床的應用做了非常好的鋪墊。 這4篇文章分別是：美國伊利
Protocolgrow up yeast culture to appropriate density (near saturation)spin 1.5 mls of culture for 1 min in microfuge and aspirate off supernatantresus
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
實驗概要 The ChargeSwitch? gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or
實驗概要The ChargeSwitch? gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. Afte
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
實驗概要The iPrep? GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
Modified Yeast Transformation Inoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically, add 0.1 to
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
· Easy YAC Preparation Method (Andrew Davies,Shaw lab)· &
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
DNA Extraction· DNA Extraction from Bacteria (Julie B. Wolf,UMBC)Phenol/chloroform method·&n
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 μg/mL) culture in early a
PCR產物的直接純化： 一、原理 PCR產物一般都含有過量的引物、 Taq DNA酶及dNTP，這些成分的存在將直接影響到后續的酶切、雙脫氧PCR測序反應等過程,因此有必要除去。目前核酸純化的方法有很多,商用化試劑盒的出現使得DNA的純化過程變得更加簡便快捷。本實驗中, Buffer PCR
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Genomic Cloning Technical ManualAn optimal strategy for genomic cloning should meet three requirements: 1) a maximum number of recombinants should be
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan