WIKI資訊
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.實驗步驟The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections......閱讀全文
實驗步驟1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated on slide substrates at 3,0
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
越來越多的研究人員開始采用小分子干擾RNA（small interfering RNAs，siRNAs)來抑制特定的哺乳動物基因表達。siRNA是一種短片斷雙鏈RNA分子，能夠以同源互補序列的mRNA為靶目標降解特定的mRNA，這個過程就是RNA干擾途徑（RNA interf
實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1. Dermatomed 0.8-mm split
Dermal Cell Suspensions 1. Dermatomed 0.8-mm split-thickness skin was obtained f
Figure 1. Silencing Gene Targets by RNase III Derived siRNA Cocktails. A 200 bp dsRNA (15 μg) for each gene of interest was digested with 2.5 U RN
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analy
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
實驗概要The following protocol provides a method of immunofluorescence of general PBMC by Peprotech.實驗步驟The following protocol used human PBMC that were
實驗概要The following protocol provides a method of immunofluorescence of mouse anti-human IL-6 by Peprotech.實驗步驟The following protocol used human endomet
實驗概要The following protocol provides a method of immunofluorescence of general cell by Peprotech.實驗步驟Please refer to the antibody Product Information S
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee
實驗概要The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monola
實驗概要The following protocol provides a method of sandwich ELISA by Peprotech.主要試劑Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liqu
Rapid detection and quantitation of total cellular acetylated proteins using our research products
實驗概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are availabl
Table 1: Hydrolysis of 125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
實驗概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (
The Kirkwood/Bloomfield CalculationThe understanding of how protein shape affects hydrodynamics is elegantly extended by an analysis originally develo
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
2 Materials2.1 Labeling1. 2′,3′-O-(2-Aminoethyl-carbamoyl)-adenosine-5′-triphosphate (edaATP) (Jena Biosciences) (see Not
GE HealthcareBenzamidine Sepharose™ 6B is p-aminobenzamidine covalentlyattached to Sepharose 6B by the epoxy coupling method.p-Aminobenzamid