Lipidanalysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer of adsorbent material which has been applied to an appropriate support. Thin layer chromatogram can be looked upon as being "open column" system. Following the development of a relatively simple apparatus for preparing chromatoplates with uniformly thin layer......閱讀全文
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Lipid－analysis：EXTRACTION－OF－LIPIDS－FROM－LIPOPHORIN
Lipid analysisEXTRACTION OF LIPIDS FROM LIPOPHORIN?Since lipids are hydrophobic, they are better soluble in organic solvents than in water. Because th
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY
During this week, you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY2
B: GLC procedure The GLC will be prepared for your use. Please do not attempt to turn on the GLC on your own if it is off. Do not change any of the
LIPID－EXTRACTION
PROCEDURE 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) 2. Add t
SREBP－control－of－lipid－synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol
Nuclear－Receptors－in－Lipid－Metabolism－and－Toxicity
Nuclear receptors are transcription factors that are activated upon binding to its ligands. Initially, they had been classified as classic endocrine n
Glycosphingolipid－analysis
1) Incubate cells with 1 μCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cy
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands?by High Performance Liquid Affinity Chromatography Analysis of Oligosaccharide Ligands by High Performance Liqui
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis.? Techniques to do this are well established in hu
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Determination－and－Detection－of－Reactive－Oxygen－Species－(ROS),－Lipid－...
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate variou
Genomic－Southern－Blot－Analysis
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis?I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA.?EmbeddingB.?CuttingC.?StainingII. Pr
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.??William Telford. Louis E. King and Pamela
PAM:－Prediction－Analysis－for－Microarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining?Features:Performs sample classificat
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose: A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspec
SOLID－TUMOR－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose: A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
實驗概要 ? ? ? ? Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. susp
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured
Lipoprotein－Analysis－Week－2:－Electrophoresis
Lipoprotein Analysis??Week 2: Electrophoresis?IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr