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Back to topReviewer CommentsReviewed by: Luis Antonio Parada, CIC Biogune, Derio, Spain.In my experience the primers are better preserved when kept at higher concentration (100μM) and different volumes are added according to the required concentrations. Usually the amplification products (unlabeled) are purified by precipitation with ethanol and dissolved in bi-distilled water at high concentration to preserve the DN......閱讀全文
Analysis of Mitochondrial Membrane Potentialwith the Sensitive Fluorescent Probe JC-1 Andrea Cossarizza and Stefano Salvioli Department of B
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Outline:To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatme
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
【摘要】 目的 探討小鼠精原細胞的分離純化。 方法 用組合酶消化法制備7～8d小鼠的生殖細胞懸液；用Percoll不連續密度梯度法分離精原細胞。 結果 所獲細胞懸液內活細胞、死細胞及細胞團的百分比分別為90.08%、9.92%及8.91%；平均每個睪丸可獲得4.136×105個細胞；精原細胞主要分布
MATERIALSMedia:Heat inactivated FBS (56°C x 30 min)PBS + 2% heat-inactivated FBSIMDM + 20% heat-inactivated FBSViral transduction: Iscove's +
實驗概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treat
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
DNA PreparationE. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol., 174, 1992). Cells are embedded in agarose, th
實驗概要The LIVE/DEAD? Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the
Introduction The most important aspect of our cloning vectors is that t
實驗概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
2B. Transformation 1. Preparation of electrocompetent DH5a cells: autoclave 4 baffled 1 l
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
B.3. COMMENTARY B.3.1 Background information The critical steps in the methodology are cell fixation, permeabilization and the concentration
Need 1.5-2 x 107 cells from a 2 day culture.1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
We use feeder-independent ES cell lines derived from the 129/Ola strain of mice (Nichols et al., Development 110, p.1341, 1990). These cells are easy
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
實驗概要Ideally, one would like to be able to directly phosphorylate substrates in an intact cell. This could potentially be performed by intr
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adh