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Back to topReviewer CommentsReviewed by: Luis Antonio Parada, CIC Biogune, Derio, Spain.In my experience the primers are better preserved when kept at higher concentration (100μM) and different volumes are added according to the required concentrations. Usually the amplification products (unlabeled) are purified by precipitation with ethanol and dissolved in bi-distilled water at high concentration to preserve the DN......閱讀全文
IntroductionMulticolour 3D-FISH in combination with confocal microscopy, 3D image reconstruction and quantitative image analysis is an efficient tool
Small DNA-probes from cosmids or plasmids clonesThese kind of probes, especially plasmids, have become out of fashion for 3D-FISH due to their delicat
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
前言今年5月，David R. Liu教授與張鋒教授等人聯合創立Beam Therapeutics公司，再一次將“單堿基編輯技術”送上了熱搜榜，趁著熱度還未散去，我們繼上一期“單堿基編輯技術淺談”后，又為大家準備了一篇超級實用性的深度分析，帶你一起看看單堿基編輯的前世今生及如何一步步發展壯大的歷程！
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis Procedure1) Prepare spleen, lymph node or T cell clone cells as
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
二、DNA標記與細胞譜系示蹤發育生物學的重點包括構成器官或生物體的細胞類型的多樣性以及這些細胞的發育譜系歷史。這些方面通常作為一個方向被單獨研究，但最近的四篇新論文報道了一種將單細胞RNA測序（scRNA-seq）技術與基于CRISPR的譜系示蹤技術相結合來同時解剖轉錄組細胞表型和譜系歷史的方法。近
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cellsZoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3,
當然，實踐是檢驗真理的唯一標準，2009年，Zoltán Ivics等[3]就為大家詳細闡述了Tol2轉座機制在鼠中的應用，具體流程如圖3，通過不斷地表型篩選，Tol2系統可以用于構建轉基因鼠和穩定的基因表達細胞系。 圖3 利用Tol2構建轉基因鼠和基因表達細胞系[3]3、Tol2轉座優勢
This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program, developed by Becton Dickinson. The
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
Figure 1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (%
In this study, we found that “acute” response was strongly induced by cryo-thermal therapy with “acute” and high expression of a series of acute p
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
實驗概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extr
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
實驗概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal? CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp