QuickandEasyIsolationofGenomicDNAfromYeast
ProcedureTransfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microcentrifuge tube. Pellet cells by centrifugation at 20,000 × g for 1-5 minutes.Add 200 μl of Harju- bufferImmerse tubes in a dry ice-ethanol bath for 2 minutes,Transfer to in a 95°C water bath for 1 minute.Repeat the last two stepsVortex 30 seconds.Add 200 μl of chloroform and ......閱讀全文
Quick－and－Easy－Isolation－of－Genomic－DNA－from－Yeast
Procedure Transfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microce
Quick－Yeast－DNA－Prep:－Isolation－of－Total－DNA－(genomic－and－plasmid)
Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg. Transfer culture to a small 13 x 100 glass tube. Spin down cells 2
Isolation－of－genomic－DNA－from－bacteria
Note: This procedure does not work well with Gram + cocci.Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, decant
Isolation－and－Quantification－of－Genomic－DNA－from－Mycobacterium－tuberculosis
Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMI
Plasmid－isolation－from－yeast
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml b
Isolation－of－Genomic－DNA－from－Tissue－Using－ChargeSwitch?－Technology
實驗概要?The ChargeSwitch? ?gDNA Mini and Micro Tissue Kits allow rapid and efficient purification ?of genomic DNA from mini (10-25 mg) or micro (3-5 mg)
酵母準備
Yeast DNA PreparationYeast Genomic Preparation? (Gottschling Lab)Rapid method for yeast genomic DNA isolation??Yeast DNA Preparation (rapid glass bead
Yeast－Genomic－DNA－Prep
Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next
DNA－Isolation－From－BAC－－PAC－Clones
DNA Isolation From BAC & PAC Clones? This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modifi
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Decontamination－of－cells－from－the－yeast
I???? Destroy yeast 1.???? Aspirate medium and wash cell in PBS. 2.???? Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic. 3.
DNA抽提
DNA抽提（主要內容如下）·???Working with DNA·???DNA Extraction from Bacteria and Other Organisms·???DNA Extraction from Cell and Tissue·???Mitochondria DNA Isola
Preparation－of－Genomic－DNA－from－Bacteria－using－Phase－Lock－GelTM
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM?(Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990
Rapid－DNA－Isolation－from－Phyllanthus－Amarus－and－Other－Plant－Tissues
Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled
Mitochondrial－DNA－Isolation－from－Somatic－Embryogenic－Cell－Cultures－of－Larix
Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) and Radetzky (1990).? Cell cultures at four d
ISOLATION－OF－RNA－FROM－BACTEROIDS
3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannit
BAC－DNA分離方法－Isolation－of－BAC－DNA－from－Largescale－Cultures
Isolation of BAC DNA from Large-scale Cultures Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne, Austral
質粒的小量制備
·?????????Standard (alkaline lysis) Mini-Prep?(Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
質粒的小量制備
·?????????Standard (alkaline lysis) Mini-Prep?(Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1.?Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone? The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97. The overall sequence of ev
Easy－YAC－Preparation－Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA [This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw,
Method:－Removal－of－Yeast－Contamination－from－Lymphoblast－Cultures
Purpose:This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when s
細菌的核酸抽提
DNA Extraction·?????????DNA Extraction from Bacteria?(Julie B. Wolf,UMBC)Phenol/chloroform method·?????????DNA Extraction From Bacteria (Triton Method
Isolation－of－osteoblasts－from－human－bone.
Bone samples were cleaned of adherent soft tissue and osteoblasts isolated by two methods.a. The bone was cut into small pieces (2 mm × 2 mm), washed
Extraction－of－DNA－using－DNAzol?－Reagent
實驗概要DNAzol? ?Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use ?reagent for the isolation of genomic DNA from solid and liquid sa
Genomic－Libraries
Genomic DNA libraries?Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bacteriu
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Yeast－DNA－Prep
Protocolgrow up yeast culture to appropriate density (near saturation)spin 1.5 mls of culture for 1 min in microfuge and aspirate off supernatantresus
RNA－Isolation－From－Animal－tissue－or－cell－culture
實驗概要This method is ?designed for most animal tissues and culture cells. For RNA isolation ?from fibrous tissue, follow the specialized protocol on pag