原代神經細胞培養方法NeuronCellCulture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those, in turn, are plated on glass coverslips pre-coated with poly-D-lysine and laminin. Materials:. #1 coverslips. coverslip racks in a water-tight container (we made ours). poly-D-lysine (PDL) stock solution (1mg/ml in dd water). laminin stock solution (20 mg/ml in Hank’s BSS). 35 mm plastic cultu......閱讀全文
原代神經細胞培養方法－Neuron－Cell－Culture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes.??Those, in turn, are plated on glass coverslips pre-
Maintenance－of－Cell－Culture
Maintenance of Cell Culture Author:?Nanci Donacki Source:?Contributed by Nanci Donacki Date Added:?Tue May 14 2002
Bacterial－cell－culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Dorsal－Root－Ganglion－Neuron－Culture－from－Adult－Rats
DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats.?ProcedurePreparing Culture Surface- Collagen co
Cell－Culture－Media－and－Solutions
Cell Culture Media and Solutions Dec. 18, 1990 R. Veile Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-gl
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 m
Cell－Suspension－Culture－of－Arabidopsis
實驗概要 Cell Suspension Culture of Arabidopsis? 主要試劑 10% (v/v) Household Bleach Callus Induction Medium? ? ? ?Gamborg's B5 Basal Medium? ? ? ?0.5
ES－Cell－Culture－and－Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Primary－brain－cell－isolation－and－culture
1.?Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Follicular－thyroid－cell－isolation－and－culture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. ?2.Kidneys and thoracic aorta were removed, rinsed with
ES－Cell－Culture－and－Manipulation3
Care and Handling of Feeder Layer Cells STO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing pl
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
A－primary－cell－culture－model－of－rabbit－uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders?1.?Animal experiments were performed in accordance with the Animal Use and Care Committee.?2.?Urina
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
MEDIA－AND－SOLUTIONS－REQUIRED－FOR－ROUTINE－ES－CELL－CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
RNA－Isolation－From－Animal－tissue－or－cell－culture
實驗概要This method is ?designed for most animal tissues and culture cells. For RNA isolation ?from fibrous tissue, follow the specialized protocol on pag
293細胞培養(cell－culture)技術
1、293細胞明顯適應酸性環境,pH值在6.9～7.1時,可順利貼壁生長, 換液時動作要輕。一般用高糖的DMEM培養基。2、傳代：倒去廢液，PBS洗一次（輕），用0.02%EDTA與0.25%Trypsin消化，生長良好細胞，培養瓶中輕搖,使之流遍所有細胞表面,即將其吸除或棄去消化30s，然后吸去，
Human－Embryonic－Stem－(ES)－Cell－Protocols——General－notes－on－ES－cell－culture
hES media has a two week shelf life. hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended be
植物細胞培養（plant－cell－culture）技術概述
植物細胞培養技術就是為了某種目的而在細胞水平上對離體植物細胞或原生質體進行的一系列生物工藝學操作。它包括分離、培養、再生以及一系列相關的操作。就有用化合物的生產來說，它主要是指在無菌條件下通過懸浮培養植物細胞生產有用化合物的過程。理論與技術基礎：植物細胞全能性、微生物液體深層發酵系統、遺傳工程
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous?flagella - the bacterial cell has a single flagellaperitrichous?flagella - the bacterial cell has several flagella w
Lactobacillus－culture
OverviewGeneral overview and guidelines on how to grow up a culture of LactobacillusMaterialsMRS broth (difco)MRS agar (difco)anaerobic conditionsProc
可靠的CCCadvanced－FN1無異源耗材支持人間充質干細...（一）
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf